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"Microfluidics for Assisted Reproduction"

Microfluidic Cumulus Removal:
Years of research went into creation and optimization of one of the most promising technologies to date.  One of the most challenging aspects of the engineering design was to create the perfectly sized, positioned and shaped ports for the intended application.  The microfluidic device, currently being tested in pre-clinical laboratory trials, is used to strip cumulus cells off of oocytes or presumptive zygotes (pending the specific ART operation). 
 

!!!PLAY THE VIDEO!!! (QuickTime)

 
Figure 1: 3D rendering of an embryo inside the microfluidic device (to scale).

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Figure 2: Image of an assembled "ready-to-use" microfluidic device.  NOTE: Green-dyed water is used for visualization purposes.

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Microfluidic Culture:
Another interesting application for microchannels is cell culture.  Media changes or additions are possible without having to "handle" the embryo.  It is also possible to perform chemical treatments by flowing the desired solution past the embryo.

FIGURE 3: Standard culture drops compared to a microfluidic channel.  The sphere is a scaled representation of an embryo "parked" at the constriction region.

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Figure 4:  A simple, one channel microfluidic culture device.

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How it Works:
  • Embryo is positioned in front of microports used for stripping of cumulus cells
  • Manipulation of the interior fluidic environment via the user causes cumulus cells to loosen and detach from the cellular complex
  • A gentle suction is applied and the loosened cumulus cells are removed from the vicinity of the embryo(s)

 

Key Features:
  • Disposable to avoid possible cross-contamination
  • 1mL sterile syringes coupled with sterile needle tips provide embryologist with precise control
  • Low media volumes used to decrease cost
  • Transparent materials used to maintain high quality imaging
  • Assembly (device and tubing) is packaged into an autoclave bag and decontaminated via steam sterilization

 
How it Works:
  • An embryo is loaded into a microchannel via a pipette
  • The embryo floats down the channel to a constriction region
  • The constriction region is smaller than the embryo so it is "parked" in one spot
  • Biologists can initiate flow, add/change media, and image the embryo

 

 
Key Features:
  • Disposable to avoid possible cross-contamination
  • Predefined, small channel geometries allow for a more in-vivo like environment
  • New culture parameters can be explored
  • Transparent materials used to maintain high quality imaging

 

Vitae LLC